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voltage dependent anion selective channel vdac  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc voltage dependent anion selective channel vdac
    Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), <t>VDAC</t> ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.
    Voltage Dependent Anion Selective Channel Vdac, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/voltage+dependent+anion+selective+channel/VDAC+Antibody/pmc12561450-49-111-118
    Average 96 stars, based on 821 article reviews
    voltage dependent anion selective channel vdac - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Comparative Analysis of the Tolerance of Young and Old Kidneys to Injury in a Rat Model of Reversible Ureteral Obstruction"

    Article Title: Comparative Analysis of the Tolerance of Young and Old Kidneys to Injury in a Rat Model of Reversible Ureteral Obstruction

    Journal: Antioxidants

    doi: 10.3390/antiox14101219

    Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), VDAC ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.
    Figure Legend Snippet: Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), VDAC ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.

    Techniques Used:

    Comparison of kidney tolerance to R-UUO-induced AKI in young and aged rats. Abbreviations: AKI—acute kidney injury; BUN—blood urea nitrogen; Col1a1—collagen type I α1 chain; cyt c—cytochrome c; Fn1—fibronectin1; IL1β—interleukin 1 beta; MMP2—matrix metalloproteinase 2; ns—no significant; PCNA—proliferating cell nuclear antigen; PGC1a—proliferator-activated receptor γ co-activator-1 alpha; SCr—serum creatinine; Tgfb1—transforming growth factor β1; Tlr2—Toll-like receptor 2; R-UUO—reversible unilateral ureteral obstruction; VDAC—voltage-dependent anion-selective channel; α-SMA—alpha-smooth actin. Created in https://BioRender.com (accessed on 7 October 2025).
    Figure Legend Snippet: Comparison of kidney tolerance to R-UUO-induced AKI in young and aged rats. Abbreviations: AKI—acute kidney injury; BUN—blood urea nitrogen; Col1a1—collagen type I α1 chain; cyt c—cytochrome c; Fn1—fibronectin1; IL1β—interleukin 1 beta; MMP2—matrix metalloproteinase 2; ns—no significant; PCNA—proliferating cell nuclear antigen; PGC1a—proliferator-activated receptor γ co-activator-1 alpha; SCr—serum creatinine; Tgfb1—transforming growth factor β1; Tlr2—Toll-like receptor 2; R-UUO—reversible unilateral ureteral obstruction; VDAC—voltage-dependent anion-selective channel; α-SMA—alpha-smooth actin. Created in https://BioRender.com (accessed on 7 October 2025).

    Techniques Used: Comparison

    Related Articles

    Incubation:

    Article Title: Disruptions to the limb muscle core molecular clock coincide with changes in mitochondrial quality control following androgen depletion
    Article Snippet: .. Membranes were then incubated overnight at 4°C with antibodies against BNIP3 (cat. no. 3769), Parkin (cat. no. 2132), LC3B (cat. no. 2775), ubiquitin-binding protein p62 (p62, cat. no. 5114), cytochrome- c oxidase subunit IV (COX IV, cat. no. 4844), voltage-dependent anion-selective channel (VDAC, cat. no. 4866), Bcl-2-like protein-1 isoform L (Bcl-xL, cat. no. 2764), glycogen synthase kinase 3β (Ser9) [GSK3β (Ser9), cat. no. 5558], total GSK3β (cat. no. 12456), Akt (Ser473) (cat. no. 4060), total Akt (cat. no. 9272), and sirtuin 1 (Sirt1, cat. no. 2028), which were all obtained from Cell Signaling Technology (Danvers, MA). ..

    Article Title: High intensity interval training induces dysregulation of mitochondrial respiratory complex and mitophagy in the hippocampus of middle-aged mice.
    Article Snippet: Autophagy is involved in aging-related cognitive impairment.. Aerobic exercise training can improve cognitive function in the elderly and this effect may be associated with autophagic mechanisms and mitochondrial respiratory function.. High intensity interval training (HIIT) has beneficial effects on heart and skeletal muscles by activating autophagy and/or mitophagy, but the effects of HIIT on autophagy/mitophagy in the aging brain are unknown.

    Ubiquitin Proteomics:

    Article Title: Disruptions to the limb muscle core molecular clock coincide with changes in mitochondrial quality control following androgen depletion
    Article Snippet: .. Membranes were then incubated overnight at 4°C with antibodies against BNIP3 (cat. no. 3769), Parkin (cat. no. 2132), LC3B (cat. no. 2775), ubiquitin-binding protein p62 (p62, cat. no. 5114), cytochrome- c oxidase subunit IV (COX IV, cat. no. 4844), voltage-dependent anion-selective channel (VDAC, cat. no. 4866), Bcl-2-like protein-1 isoform L (Bcl-xL, cat. no. 2764), glycogen synthase kinase 3β (Ser9) [GSK3β (Ser9), cat. no. 5558], total GSK3β (cat. no. 12456), Akt (Ser473) (cat. no. 4060), total Akt (cat. no. 9272), and sirtuin 1 (Sirt1, cat. no. 2028), which were all obtained from Cell Signaling Technology (Danvers, MA). ..

    Saline:

    Article Title: High intensity interval training induces dysregulation of mitochondrial respiratory complex and mitophagy in the hippocampus of middle-aged mice.
    Article Snippet: Autophagy is involved in aging-related cognitive impairment.. Aerobic exercise training can improve cognitive function in the elderly and this effect may be associated with autophagic mechanisms and mitochondrial respiratory function.. High intensity interval training (HIIT) has beneficial effects on heart and skeletal muscles by activating autophagy and/or mitophagy, but the effects of HIIT on autophagy/mitophagy in the aging brain are unknown.

    Binding Assay:

    Article Title: High intensity interval training induces dysregulation of mitochondrial respiratory complex and mitophagy in the hippocampus of middle-aged mice.
    Article Snippet: Autophagy is involved in aging-related cognitive impairment.. Aerobic exercise training can improve cognitive function in the elderly and this effect may be associated with autophagic mechanisms and mitochondrial respiratory function.. High intensity interval training (HIIT) has beneficial effects on heart and skeletal muscles by activating autophagy and/or mitophagy, but the effects of HIIT on autophagy/mitophagy in the aging brain are unknown.

    Pyrolysis Gas Chromatography:

    Article Title: A chronic low-dose magnesium L-lactate administration has a beneficial effect on the myocardium and the skeletal muscles.
    Article Snippet: .. The membranes were then immunoblotted with the appropriate antibody to detect acetylated superoxide dismutase 2 (Ac-SOD2, 24 kDa, 1:1000, Abcam #ab137037), calcium calmodulin ATPase II beta/gamma/delta (CaMKII, 47 kDa, 1:1000, GeneTex #GTX52374), calcium calmodulin ATPase II beta/gamma/delta phosphorylated at threonine 287 (CaMKIIP, 47 kDa, 1:1000, GeneTex #GTX523424), glutathione peroxidase-1 (GPX1, 22 kDa, 1 μg/ml, Abcam #ab22604), sodium/proton exchanger 1 (NHE1, 91 kDa, 1:500, GeneTex #GTX85047), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α, 90 kDa, 1:500, Santa Cruz sc-13067), pyruvate dehydrogenase (PDH, 43 kDa, 1 μg/ml, Abcam #ab110330), pyruvate dehydrogenase phosphorylated at serine 293 (PDHP, 40 kDa, 1/1000, Abcam #ab177461), superoxide dismutase 2 (SOD2, 22 kDa, 1:1000, Cell Signaling #13,194) and voltage-dependent anion-selective channel (VDAC, 32 kDa, 1:1000, Cell Signaling #4866). .. Antibody binding was detected using horseradish peroxidase (HRP)-conjugated secondary antibodies and the ECL western blotting substrate (Thermo Fisher Scientific, Asnières-sur-Seine, France).

    Article Title: EPA Is Cardioprotective in Male Rats Subjected to Sepsis, but ALA is Not Beneficial
    Article Snippet: .. The membranes were then immunoblotted with the appropriate antibody to detect acetylated-superoxide dismutase 2 (Ac-SOD2, 24 kDa, 1:1000, Abcam #ab137037), nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor alpha (IκBα, 39 kDa, 1:1000, Cell Signaling #9242), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α, 90 kDa, 1:500, Santa Cruz sc-13067), superoxide dismutase 2 (SOD2, 22 kDa, 1:1000, Cell Signaling #13194), uncoupling protein-3 (UCP3, 34 kDa, 1:1000, Abcam #ab10985), and voltage-dependent anion-selective channel (VDAC, 32 kDa, 1:1000, Cell Signaling #4866). .. Antibody binding was detected using horse raddish peroxidase (HRP)-conjugated secondary antibodies and the ECL Western Blotting Substrate (Thermo Fisher Scientific, Asnières-sur-Seine, France).



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    Image Search Results


    Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), VDAC ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.

    Journal: Antioxidants

    Article Title: Comparative Analysis of the Tolerance of Young and Old Kidneys to Injury in a Rat Model of Reversible Ureteral Obstruction

    doi: 10.3390/antiox14101219

    Figure Lengend Snippet: Assessment of the level of key OXPHOS proteins in the young and old kidneys. Protein level of cox I ( A ), cox IV ( B ), cyt c ( C ), VDAC ( D ), ATP synthase subunit b ( E ), and acetylated H3 histone ( F ). The data for young rats are shown in green, and the data for old rats are shown in orange. * p < 0.05, ** p < 0.01, *** p < 0.001 statistically significant differences (two-way ANOVA, Dunnett’s post hoc test). The examples shown are representative of at least 5 independent experiments.

    Article Snippet: Subsequently, they were incubated with primary antibodies diluted in 0.1% bovine serum albumin (BSA), against alpha-smooth muscle actin (α-SMA) 1:1000 (ab5694, Rb, Abcam, Cambridge, UK), proliferating cell nuclear antigen (PCNA) 1:1000 (13110, Rb, Cell Signaling, Boston, MA, USA), Acetyl-Histone H3 (Lys9) 1:1000 (9649, Rb, Cell Signaling, Boston, MA, USA), beclin-1 1:1000 (3495, Rb, Cell Signaling, Boston, MA, USA), Sequestosome-1 (SQSTM1)/p62 1:1000 (5114, Rb, Cell Signaling, Boston, MA, USA), complex IV 1:1000 (ab109863, Ms, Abcam, Cambridge, UK), complex I 1:1000 (A21344, Ms, Thermofisher Scientific, Carlsbad, CA, USA), cytochrome c (cyt c) 1:1000 (556433, Ms, BD Biosciences, San Jose, CA, USA), ATP synthase subunit b 1:1000 (MABS1304, Ms, Millipore, St. Louis, MO, USA), voltage-dependent anion-selective channel (VDAC) 1:1000 (4866, Rb, Cell Signaling, Boston, MA, USA), and β-actin 1:2000 (A2228, Ms, Sigma-Aldrich, St. Louis, MO, USA) at 4 °C overnight.

    Techniques:

    Comparison of kidney tolerance to R-UUO-induced AKI in young and aged rats. Abbreviations: AKI—acute kidney injury; BUN—blood urea nitrogen; Col1a1—collagen type I α1 chain; cyt c—cytochrome c; Fn1—fibronectin1; IL1β—interleukin 1 beta; MMP2—matrix metalloproteinase 2; ns—no significant; PCNA—proliferating cell nuclear antigen; PGC1a—proliferator-activated receptor γ co-activator-1 alpha; SCr—serum creatinine; Tgfb1—transforming growth factor β1; Tlr2—Toll-like receptor 2; R-UUO—reversible unilateral ureteral obstruction; VDAC—voltage-dependent anion-selective channel; α-SMA—alpha-smooth actin. Created in https://BioRender.com (accessed on 7 October 2025).

    Journal: Antioxidants

    Article Title: Comparative Analysis of the Tolerance of Young and Old Kidneys to Injury in a Rat Model of Reversible Ureteral Obstruction

    doi: 10.3390/antiox14101219

    Figure Lengend Snippet: Comparison of kidney tolerance to R-UUO-induced AKI in young and aged rats. Abbreviations: AKI—acute kidney injury; BUN—blood urea nitrogen; Col1a1—collagen type I α1 chain; cyt c—cytochrome c; Fn1—fibronectin1; IL1β—interleukin 1 beta; MMP2—matrix metalloproteinase 2; ns—no significant; PCNA—proliferating cell nuclear antigen; PGC1a—proliferator-activated receptor γ co-activator-1 alpha; SCr—serum creatinine; Tgfb1—transforming growth factor β1; Tlr2—Toll-like receptor 2; R-UUO—reversible unilateral ureteral obstruction; VDAC—voltage-dependent anion-selective channel; α-SMA—alpha-smooth actin. Created in https://BioRender.com (accessed on 7 October 2025).

    Article Snippet: Subsequently, they were incubated with primary antibodies diluted in 0.1% bovine serum albumin (BSA), against alpha-smooth muscle actin (α-SMA) 1:1000 (ab5694, Rb, Abcam, Cambridge, UK), proliferating cell nuclear antigen (PCNA) 1:1000 (13110, Rb, Cell Signaling, Boston, MA, USA), Acetyl-Histone H3 (Lys9) 1:1000 (9649, Rb, Cell Signaling, Boston, MA, USA), beclin-1 1:1000 (3495, Rb, Cell Signaling, Boston, MA, USA), Sequestosome-1 (SQSTM1)/p62 1:1000 (5114, Rb, Cell Signaling, Boston, MA, USA), complex IV 1:1000 (ab109863, Ms, Abcam, Cambridge, UK), complex I 1:1000 (A21344, Ms, Thermofisher Scientific, Carlsbad, CA, USA), cytochrome c (cyt c) 1:1000 (556433, Ms, BD Biosciences, San Jose, CA, USA), ATP synthase subunit b 1:1000 (MABS1304, Ms, Millipore, St. Louis, MO, USA), voltage-dependent anion-selective channel (VDAC) 1:1000 (4866, Rb, Cell Signaling, Boston, MA, USA), and β-actin 1:2000 (A2228, Ms, Sigma-Aldrich, St. Louis, MO, USA) at 4 °C overnight.

    Techniques: Comparison